Ready-to-Use Multiplex Assays: Twelve qPCR vials (5-plex format) optimized for consistent and sensitive amplification.
Built-in Internal Amplification Control (Cy5.5): Every 5-plex mix includes an Internal Control detected on the Cy5.5 channel, ensuring real-time monitoring of amplification performance and PCR inhibition.
Positive Control: Whole genome libraries Normalized to 500 genomic copies/ul
Exogenous Control (EC): Spike-in Non-Pathogen Whole organism controls to confirm extraction and amplification efficiency.
Comprehensive Documentation: Includes detailed protocols, troubleshooting guide, and Certificate of Analysis (COA).
Comprehensive Detection of ocular pathogens and associated resistance markers
- Bacterial Targets: Acinetobacter baumannii, Citrobacter freundii / braakii / koseri, Cutibacterium (Propionibacterium) acnes, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Haemophilus influenzae, Klebsiella oxytoca, Klebsiella pneumoniae, Klebsiella (Enterobacter) aerogenes, Moraxella catarrhalis, Neisseria gonorrhoeae, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus agalactiae (Group B), Streptococcus pneumoniae, Streptococcus pyogenes (Group A)
- Viral Targets: Human Adenovirus D, Human Gammaherpesvirus 4 (Epstein-Barr virus, EBV), Human Cytomegalovirus (Human Herpesvirus 5, CMV), Herpes Simplex Virus 1 & 2, Varicella Zoster Virus (Human Herpesvirus 3, VZV), Rhinovirus / Enterovirus (including EV68 & EV71)
- Fungal Targets: Aspergillus spp., Candida spp. (C. albicans, C. dubliniensis, C. parapsilosis, C. tropicalis), Candida glabrata, Fusarium spp.
- Parasitic / Protozoan Targets: Acanthamoeba spp., Vermamoeba (Hartmannella) vermiformis, Microsporidia (Encephalitozoon hellem)
- AMR: AmpC (ACT / MIR / FOX), Beta-lactamase (CTX-M Group 1, SHV), Carbapenem resistance (OXA), Carbapenemase (NDM / KPC), Fluoroquinolone resistance (qnrA / qnrB / qnrS), Methicillin resistance (mecA), Macrolide resistance (ermB / ermC, mefA), Sulfonamide resistance (sul1), Tetracycline resistance (tetB / tetM), Trimethoprim resistance (dfrA1), Vancomycin resistance (VanA / VanB / VanM)
- Controls: Internal Control (RNase P), Exogenous Control (EC)
Covers 21 bacterial targets, 6 viral targets, 4 fungal targets, 3 parasitic/protozoan targets, 12 antimicrobial resistance markers, and 2 internal controls (48 total targets), enabling comprehensive multi-etiology pathogen detection and antimicrobial resistance profiling.
