Ready-to-Use Multiplex Assays: Twelve qPCR vials (5-plex format) optimized for consistent and sensitive amplification.
Built-in Internal Amplification Control (Cy5.5): Every 5-plex mix includes an Internal Control detected on the Cy5.5 channel, ensuring real-time monitoring of amplification performance and PCR inhibition.
Positive Control: Whole genome libraries Normalized to 500 genomic copies/ul
Reverse Transcription Control (RTC): Internal RNA spike-in to confirm extraction, reverse transcription, and amplification efficiency.
Comprehensive Documentation: Includes detailed protocols, troubleshooting guide, and Certificate of Analysis (COA).
Comprehensive Detection of gastrointestinal pathogens and associated resistance markers
- Bacterial Targets: Clostridioides (Clostridium) difficile, Enteroaggregative Escherichia coli (EAEC), Enterotoxigenic Escherichia coli (ETEC), Escherichia coli O157:H7 (EHEC), Shiga toxin–producing E. coli (STEC; stx1/stx2), Helicobacter pylori, Salmonella enterica subsp. enterica serovar Typhimurium, Shigella spp. / Enteroinvasive E. coli (EIEC), Campylobacter (C. jejuni, C. coli, C. fetus, C. lari, C. upsaliensis), Vibrio spp. (V. parahaemolyticus, V. vulnificus, V. cholerae), Yersinia enterocolitica (biotypes 1B/2/3)
- Viral Targets: Norovirus GI, Norovirus GII, Rotavirus A, Rotavirus B, Sapovirus (Caliciviridae), Human adenovirus type 40/41 (Adenovirus F), Human astrovirus (Astroviridae)
- Fungal Targets: Aspergillus spp., Candida spp. (C. albicans, C. dubliniensis, C. parapsilosis, C. tropicalis), Candida glabrata, Fusarium spp.
- Parasitic / Protozoan Targets: Entamoeba histolytica, Giardia lamblia (G. intestinalis / G. duodenalis), Cryptosporidium hominis / parvum, Ancylostoma duodenale, Necator americanus, Enterobius vermicularis
- AMR: Sulfonamide resistance (sul1), Vancomycin resistance (VanA / VanB / VanM), Fluoroquinolone resistance (qnrA / qnrB / qnrS), Tetracycline resistance (tetB / tetM), Trimethoprim resistance (dfrA1), Macrolide resistance (mefA/E, erm)
- Controls: Internal Control (RNase P), Reverse Transcription Control (RTC)
Covers 21 bacterial targets, 6 viral targets, 4 fungal targets, 3 parasitic/protozoan targets, 12 antimicrobial resistance markers, and 2 internal controls (48 total targets), enabling comprehensive multi-etiology pathogen detection and antimicrobial resistance profiling.
